Ụdị Circovirus Porcine -Ⅱ Ngwa nchọpụta ELISA Antibody
Nọmba nlereanya:VD510E
Abbr.:PCV{1}}Ⅱ Ab Elisa
Ụdị: serum
Nkọwa
Nkọwapụta
PCV{0}Ⅱ Ab Elisa
Ezubere iji:
Ụdị Porcine Circovirus -Ⅱ Ngwa nchọpụta ELISA Antibody bụ maka mkpebi mgbochi PCV kpọmkwem na serum pig in vitro. A na-eji ule a chọpụta ọgwụ ịgba ọgwụ mgbochi ọrịa na ụdị porcine circovirus -Ⅱ nke ugbo ezi.
Ụdị:ọbara
Ihe ndị enyere:
Usoro ule & Nkọwa
Ntụpụ ụkwụ 1 Usoro nnwale
1.1 Nkwadebe ngwọta saa
Na-arụ ọrụ ịta ahụhụ na-asa mmiri mmiri: Tupu eji ya, a ga-eweghachi ihe ngwọta na-asa ahụ na okpomọkụ (20-25 degrees) ma maa jijiji nke ọma iji mee ka ọ bụrụ otu. Mgbe ahụ, 20 ugboro 20 ihe ngwọta na-etinye uche na mmiri dị ọcha (dịka ọmụmaatụ, a na-agbakwunye 5ml na-asachapụ na mmiri dị ọcha 95ml), gwakọta nke ọma, na mmiri mmiri a na-asachapụ nwere ike ịchekwa na 2-8 degrees maka ụbọchị 7.
1.2 Nkwadebe ihe atụ
① E were ọbara anụmanụ dum, gbachie ya na 4000rpm maka nkeji iri, wee chịkọta ihe ndị dị elu. Enwere ike imechi ọbara n'onwe ya na-enweghị hemolysis.
② Ghichaa ihe nlele ọbara 1: 25 (tinye 5ul serum sample na 120ul sample diluent) ma gwakọta nke ọma.
Rịba ama: njikwa adịghị mma na nke dị mma adịghị mkpa ka e megharịa ya.
1.3 Enzyme conjugate Nkwadebe
Gwakọta enzyme conjugate 1:25 (tinye 5ul enzyme conjugate na 120ul enzyme diluent) ma gwakọta nke ọma.
1.4 Usoro ule
① Were enzyme label Microplate (dị ka ọnụ ọgụgụ nke samples, nwere ike disassembled na-eji ọtụtụ ugboro), tinye 100ul nke diluted serum na enzyme labelụ Microplate, set abụọ olulu mmiri ọ bụla maka adịghị mma na nti samples, na tinye 100ul ọ bụla maka ọjọọ na nti njikwa na mmeghachi omume nke ọma karị. Jiri nwayọọ na-akwagharị ihe nlele ahụ na olulu mmiri ahụ, jiri ihe nkiri mkpuchi mkpuchi kpuchie efere ahụ, ma tinye ya na ogo 37 maka nkeji 30.
② Mepee ihe nkiri efere akara, kpochapụ ihe ngwọta na efere ahụ nke ọma, tinye 300µl nke mmiri ịsa ahụ na-arụ ọrụ na olulu mmiri ọ bụla, wee tụfuo mmiri mmiri n'ime olulu mmiri ahụ. Tinyegharịa usoro ndị a dị n'elu ugboro 5, ma n'ikpeazụ kpochaa na mpempe akwụkwọ dị ọcha.
③Tinye 100µl nke Enzyme Conjugate na olulu mmiri ọ bụla, kpuchie efere ahụ, tinye ya na ogo 37 maka nkeji 45.
④ Mepee ihe nkiri efere akara, kpochapụ ihe ngwọta na efere ahụ nke ọma, gbakwunye 300µl nke mmiri ịsa ahụ na-arụ ọrụ na olulu mmiri ọ bụla, wee tụfuo mmiri mmiri na olulu mmiri. Tinyegharịa usoro ndị a dị n'elu ugboro 5, ma n'ikpeazụ kpochaa na mpempe akwụkwọ dị ọcha.
⑤ Tinye ihe ngwọta 100µl na olulu mmiri ọ bụla, kpuchie efere ahụ ma tinye ya maka nkeji 10 na ogo 37 pụọ na ìhè.
⑥ Tinye 50µl ngwọta nkwụsị na olulu mmiri ọ bụla, ma chọpụta nsonaazụ n'ime nkeji 5.
Ntụpụ ụkwụ 2 Nkọwa nsonaazụ
A na-agụ uru OD450 nke olulu mmiri ọ bụla na onye na-agụ microplate dị 96.
2.1 nkwado
Nkezi uru OD450nm nke njikwa adịghị mma bụ<0.1 and the average OD450nm value of positive control /the average OD450nm value of negative control was ≥5, the experimental results were valid. Otherwise, the test should be repeated.
2.2 Usoro mgbako
Maka nlele ọ bụla, gbakọọ S/P dị ka ndị a site na iji nlele na njikwa njikwa:
S / P{0} (ODNlereanya-ODNC) / (ODPC-ODNC)
2.3 Nkọwapụta nsonaazụ
Mgbe S/P<0.15, it is judged as negative.
Mgbe S/P Karịrị ma ọ bụ nhata 0.25, a na-ekpebi ya dị ka ihe dị mma.
Mgbe 0.15 Na-erughị ma ọ bụ hà nhata S/P<0.25, it is judged as suspicious, and suggest to repeat sampling and test one week later.
Ihe nchịkọta dị ọkụ: porcine circovirus ụdị -ⅱ ngwa nchọpụta elisa antibody, China porcine circovirus ụdị{1}}ⅱ ngwa ihe nchọpụta ihe mgbochi elisa ndị na-ebubata, ndị na-emepụta ihe, ụlọ ọrụ mmepụta ihe
Nke gara aga: Enweghị Ozi
